Fig. 3

MTA1 is exported from apoptotic cells in a caspase dependent manner. (A) Fluorescence microscopy images showing SiHa cells co-expressing GFP-MTA1 and RFP-Coilin, taken 24 h after 16 Gy lethal irradiation (IR). (B) Immunofluorescence staining of CC cells, with MTA1 labeled in green and phosphorylated histone H2AX in red. (C) Fluorescence images of CC cells co-expressing GFP-MTA1 and RFP-NLS following IR treatment. (D) Western blot analysis of EVs isolated from CC cells subjected to IR for different time points, with or without the pan-caspase inhibitor zVAD(OMe)fmk