Fig. 1

MTA1 is highly expressed in apoptotic CC cells and correlates with radioresistance of CC patients. (A) Assessment of caspase-3 activity using FACS analysis in six freshly dissociated tumor samples. (B) Gene ontology enrichment analysis of 12 differentially expressed genes from the GSE236738 public dataset. (C) Identification of MTA1, QRICH1, and ATF4 as potential radioresistance regulators through Venn diagram analysis across KEGG, Metascape, and STRING platforms. (D) RT-qPCR analysis comparing the RNA expression levels of MTA1, QRICH1, and ATF4 in radiotherapy-sensitive and resistant CC tissues. (E) Western blot analysis showing the protein expression levels of MTA1, QRICH1, and ATF4 in radiotherapy-sensitive and resistant CC tissues. (F) TCGA data indicating that MTA1 overexpression is associated with poor prognosis in CC patients (*P = 0.0319, log-rank test). (G) Representative IHC staining images of caspase-3 and MTA1 in radiotherapy-sensitive and resistant CC tissues at 200× magnification (scale bar, 50 μm). (H) Statistical analysis of caspase-3 and MTA1 expression levels (*P < 0.05). (I) Pearson correlation analysis showing a significant clinical correlation between caspase-3 and MTA1 (r = 0.6870, P < 0.001)