Fig. 6
YTHDF1 facilitates BAIAP2L2 translation through m6A modification. A GSEA plot for the pathways associated with the regulation of transcription by RNA polymerase II in drug-resistance group. B-C qPCR, western blot and IF (Scale bars were 20 μm) were used to explore the regulation effect of YTHDF1 on BAIAP2L2. D Western Blot showed the expression of YTHDF1 in DDP-resistant GC cells and DDP-sensitive cells. E Methylated RNA immunoprecipitation of the transcripts of BAIAP2L2 in AGS, MKN45 and MKN45/DDP. F YTHDF1 immunoprecipitation assays of BAIAP2L2 transcripts in YTHDF1-bound mRNAs in MKN45 and MKN45/DDP. G CCK-8 were used to explore the effect of YTHDF1 on the resistance to platinum chemotherapeutics of GC cells. Data of CCK-8 assays were analyzed by 2-way ANOVA. H–I IHC staining were performed to detect the expression of YTHDF1 in GC tissues of patients resistant or sensitive to Platinum-based treatment. Scale bars for 20 × figures are 100 μm, and those for 40 × figures are 50 μm. The P value was calculated with the chi-square test (χ2). J The subcutaneous tumors from the BALB/c Nude mice injected LV-Ctrl or LV-ythdf1 MKN45/DDP cells, with DDP treatment (DDP 5 mg/kg once per week). K Weight and volume of subcutaneous tumors. Data on subcataneous tumor volume were analyzed by 2-way ANOVA. L–M IHC staining of the subcutaneous tumors was used to confirmed the regulation effect of YTHDF1 on BAIAP2L2.Scale bars for 20 × figures are 100 μm, and those for 40 × figures are 50 μm. The P value was calculated with the chi-square test (χ2)