Fig. 5

ANXA4 promotes platinum-based chemical resistance in GC by mediating autophagy. A qPCR showed the expression of ANXA4 in EVs from BAIAP2L2 knock-down MKN45/DDP and in the control group. B CCK-8 were used to explore the counteraction of pre-knockdown of ANXA4 in MKN45 cells to the effect of EVs isolated from BAIAP2L2 overexpression MKN45/DDP. C-E The subcutaneous tumors from the BALB/c Nude mice injected LV-Ctrl or LV-ANXA4 MKN45 sensitive cells, with DDP treatment (DDP 5 mg/kg once per week), with 20 μg of different EVs (resuspended in 40 μL of PBS) injected through the caudal vein as once per week. C Flow diagram of Flow diagram of Subcutaneous tumor model. D Volume of subcutaneous tumors. Data on subcataneous tumor volume were analyzed by 2-way ANOVA. J Flow diagram of the subcutaneous tumor xenograft experiment in BALB/c Nude mice. F Western Blot showed the expression of ANXA4 in DDP-resistant GC cells and DDP-sensitive cells. G, H Western Blot and IF showed the expression of LC3 and p62 levels in ANXA4 overexpression MKN45 and the control group. I TEM images of ultrastructure microstructure in representative overexpression ANXA4 and control group