Fig. 4

BAIAP2L2 promotes the transfer of chemotherapy resistance from resistant GC cells to sensitive cells through EVs. A Representative transmission electron microscope image (scale, 100 nm) of EVs derived from knocked-down or control BAIAP2L2 MKN45/DDP cells. B NTA were performed to show the size of purified EVs derived from knocked-down or control BAIAP2L2 MKN45/DDP cells (nm). C Flow diagram of EV labeling and co-culture. D EVs derived from overexpression or control BAIAP2L2 MKN45/DDP stained with PKH67 fused into sensitive MKN45 cells. E EVs markers (HSP70, TSG101, CD9 and CD63) and BAIAP2L2 in knocked-down or control BAIAP2L2 MKN45/DDP cell proteins and corresponding EVs were analyzed by Western Blot. F CCK-8 were used to explore the effect of EVs isolated from knocked-down or overexpression BAIAP2L2 MKN45/DDP. G-H Top ten molecules select from down-regulated proteins according to logFC in TMT analysis. I CCK-8 were used to confirm that ANXA4 could promote chemotherapy resistance