Fig. 2

Data quality and α and β diversity of microbial sequences. A The abscissa represents the length of the sequence, and the ordinate represents the number of sequences. The closer the length of the sequence obtained by sequencing to the length of the 16s rRNA amplified region, the better the amplification specificity. B Venn diagram shows the number of unique and common OUTs in EOC, TB, and Nor groups. C Heat map of OTUs distribution in samples from each group. D Ace, Simpson, Shannon, and Chao1 indexes of the three groups were compared by Wilcoxon test between the two groups and Kruskal–Wallis test between the three groups. “*” represented p < 0.05, “**” represented p < 0.01. E PLS-DA analysis of intestinal flora among the three groups. F PLS-DA model test plot