Fig. 6

Wnt5a upregulates free cholesterol levels and activates AR signaling. (A) Enzymatic assays of total and free cholesterol in LNCaP and (B) 22RV1 cells with different Wnt5a expression levels. (C) Filipin staining was used to detect the content of free cholesterol in the indicated cells. (D) RT-qPCR was performed to detect the effect of Wnt5a on the expression levels of AR target genes in LNCaP and (E) 22RV1 cells. Data are presented as mean ± SD. *P < 0.05; **P < 0.01; ns, not significant