Fig. 6

BMP9 treatment did not significantly impact osteoclastogenesis or bone resorption. A TRAP staining results are shown for different experimental groups. TRAP staining is primarily used to label osteoclasts. The results indicated a significant increase in osteoclast number in the CIA group, while BMP9 treatment did not significantly affect osteoclast numbers. B The number of osteoclasts (Oc.N) in each group is shown. In the BMP9 group, the osteoclast count was not significantly different from the CIA or CIA + GFP groups (p > 0.05), suggesting that BMP9 did not significantly influence osteoclast formation or quantity. C Measurement of bone resorption surface (ES/BS) is shown. There was no significant difference in the ES/BS between the BMP9 group and the CIA or CIA + GFP groups (p > 0.05), indicating that BMP9 did not significantly improve bone resorption. D Measurement of osteoclast surface area (OC.s/BS) is shown. The osteoclast surface area in the BMP9 group did not differ significantly from that in the CIA or CIA + GFP groups (p > 0.05), further supporting that BMP9 did not significantly affect osteoclast formation