Fig. 5

Effects of ferroptosis on citrullinated antigens, endogenous antigen presenting markers and inflammatory factors

(a) Image of hematoxylin and eosin staining of hind knee joint; (b-d) Histological scores of joint inflammation, synovial bone erosion and hyperplasia; (e-f) Representative immunohistochemical staining of CD8, MHC-I, cit-h3, PAD4, TNF-α and IFN-γ and quantitative analysis of the percentage of positive cells. (n = 3); NC = normal control; PBS = CIA mice group; Erastin = CIA mice treated with erastin group. Fer-1 = CIA mice treated with fer-1 group. Erastin + GSK484 = CIA mice treated with erastin and GSK484 group. Data are expressed as mean ± S.D. Each dot plot represents an individual sample; For statistical analysis, one-way ANOVA followed by Dunnett’s method for multiple comparisons. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001