Fig. 1
Ferroptosis and increased MHC-I levels in rheumatoid arthritis
(a, b) qPCR and WB analyse the expression of GPX4 in FLSs and the statistical result of the relative fold values (normalized the OA control group) (n = 5–6); (c) Flow cytometry analysis of FLSs after staining with 1 µM C11-BODIPY581/591 (n = 3–5); (d) Immuno-fluoresence of FLSs stained for DCFH-DA and the statistical result of mean fluorescence intensity (MFI) (n = 4–6); (e) Immuno-fluoresence of FLSs stained for FerroOrange (green) and the statistical result of mean fluorescence intensity (MFI) (n = 5); (f, g) qPCR and WB analyse the expression of HLA-I in FLSs and the statistical result of the relative fold values (normalized the OA control group) (n = 6–7); (h) Flow cytometry analysis the expression of HLA-A2 in FLS(n = 5); (i) Flow cytometry analysis the expression of cit-h3 in FLSs (n = 4); Data are shown as the mean ± S.D.; Each dot plot represents an individual sample; All data were statistically analyzed using Mann-Whitney U test. *P < 0.05, **P < 0.01, ***P < 0.001