Fig. 3
Association of cell state abundance with patient outcomes across different groups A Survival associations of 66 cell states in the TCGA-HNSC cohort, with the most significant marker genes for both unfavorable and favorable cell states indicated. If a cell state is associated with shorter survival, the survival association is represented as the -log10(p-value) multiplied by 1. For cell states associated with favorable outcomes, it is shown as −1. B Scatter plot illustrating the correlation between cell state survival associations in the TCGA-HNSC cohort and the GEO integrated cohorts (GSE41613, GSE42743, GSE65858). Spearman correlation coefficients and two-sided P-values are indicated. The graph also displays the linear regression best-fit line and the 95% confidence interval. C Kaplan-Meier survival curves for macrophages (S06) and plasma cells (S08) in the TCGA-HNSC cohort. D Representative multicolor immunofluorescence image of ACTA2 + fibroblast. E Prognostic curves for ACTA2 + fibroblast across different cohorts