Fig. 6

LncFLJ prevents AR from entering the nucleus and stabilizes its protein in the cytoplasm. A The expression of AR and target proteins following FLJ knockdown was assessed by WB. B The expression of AR and target genes following FLJ knockdown was evaluated by qRT-PCR. C The lncLocator website was used to predict the subcellular localization of FLJ. D The subcellular localization of FLJ in 22Rv1 cells was assessed by RNA-FISH, with a scale bar of 50 μm. E The subcellular localization of FLJ in 22Rv1 cells after RNA nuclear and cytoplasmic separation was evaluated by qRT-PCR. F, G The subcellular distribution of AR after FLJ knockdown was assessed by immunofluorescence, with a scale bar of 25 μm. H The subcellular distribution of AR and target proteins after nuclear and cytoplasmic separation was assessed by WB. I The regulation of cytoplasmic AR protein degradation rate following FLJ knockdown was evaluated by WB