Fig. 10

The downregulation of AC129507.1 activates ferroptosis. A The expression levels of lipid metabolism-related genes were detected by RT‒PCR. B The expression levels of glycolysis enzyme-related genes were detected by RT‒PCR, and β-actin was used as an internal control. C The expression levels of ferroptosis-related genes were detected by RT‒PCR. D The levels of the GPX4, SLC7A11, FTH1, NRF2, NCOA4, SOD1 and SOD2 proteins were detected using western blotting. GAPDH served as an internal control. E Malondialdehyde (MDA) levels were detected after AC129507.1 was knocked down in AGS and HGC27 cells. F Intracellular ROS levels were detected in AC129507.1-knockdown GC cells. G Apoptosis was detected in AC129507.1-knockdown GC cells by flow cytometry