Fig. 5

Single cell sequencing analysis revealed the effect of MCT4 knockout on tumor microenvironment. A Unsupervised cluster TSNE of single cell sequencing results of liver and tumor in mice with HGC-27 cells modeled after MCT4 knockout. B Bubble map of marker genes for identification of cell types. C Unsupervised clustering TSNE plots of KO group and NC group. D Histogram of cell composition in KO group and NC group. E–G Statistical violin diagram of differences in immune activation, tumor inhibition and chemokine-related levels of different cell subsets. H, I Differential function of neutrophil subsets in different subgroups KEGG and GO enrichment analysis Bubble map