Fig. 6

Silencing of PRDM10-DT or SP1 can decrease X-ray-induced tumor angiogenesis in vivo. A Graphical illustration of the subcutaneous A549 tumor model (SC injection), X-ray irradiation, and drug treatment (created with BioRender.com). B Photograph of dissected tumors described in A. C Subcutaneous tumor growth curve after 40 days of treatment. n = 5. D The weights of the dissected tumors were measured as described in A. n = 5. E The levels of TGF-β1 and VEGF in the serum of the mice described in A were tested via ELISA. n = 5. F The dissected tumor tissues were processed into sections and then subjected to H&E or IHC staining. The graphs of these sections were taken via the DMS-10 Digital Pathological Section Scanner System. Scale bar = 50 μm. The results of the IHC quantification of CD31, Ki67, VEGF, TGF-β1, and SP1 expression and the MVD of the dissected tumor tissues were analyzed with ImageJ software. The yellow dotted line indicates microvessels in the tumor sections. n = 5. The data are presented as the means ± standard deviations (SDs). ^ns p > 0.05; * p < 0.05; ** p < 0.01; and *** p < 0.001