Fig. 1

PRDM10-DT is a prognostic risk factor for X-ray-induced lung metastasis. A 2 Gy X-ray- or C-ion-irradiated NSCLC tumor cells were injected intravenously into the mice, and the luciferase intensity at the metastasis sites was checked via an IVIS imaging system after 3 weeks. The lung tissues were isolated and photographed and then processed into sections for metastasis site determination. n = 6. B,C Quantification of metastasis sites and HE-stained images of lung tissues. n = 6. D Heatmap showing the differentially expressed lncRNAs in X-ray- or C-ion-irradiated NSCLC samples (p < 0.05, FC > 2.0). E Volcano plot displaying the significantly upregulated or downregulated lncRNAs described in D after irradiation. The blue and red dots indicate significantly changed genes (p < 0.05, FC > 2.0). F The lnCAR online database provides the PRDM10-DT expression levels in NSCLC tumor and normal tissues. G The survival curves of NSCLC patients with different expression levels of PRDM10-DT were retrieved from the OncoLnc database. H GSEA results showing the enrichment of the differentially expressed mRNAs affected by PRDM10-DT in the TGF-β signaling pathway after X-ray or C-ion irradiation. The data are presented as the means ± standard deviations (SDs). ^ns p > 0.05; * p < 0.05; ** p < 0.01