Fig. 8

The knockdown of Tregs reduces the protective effect of PBP-EVs on AKI. (A) Schematic diagram of the Foxp3-DTR mouse. (B) Real-time qPCR of renal inflammation-related genes (TGF-β, IL-6, TNF-α and IL-10) in each group. n = 3. (C) The level of SCr and BUN on days 3 after IRI. n = 3. (D) H&E staining of renal tissue at day 3 after IRI. Scale bar, 100 μm. (E) α-SMA immunofluorescence staining at day 28 after IRI (α-SMA, red). Scale bar, 100 μm. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparison tests. All data are expressed as the mean ± s.d. ^*P < 0.05