Fig. 6

IPA changes cell size and cytoplasm complexity in LX-2 cells. A Representative images from flow cytometry analysis. The analysis was performed using a gating strategy for LX-2 cells: SSC-A/FSC-A determination of cell population, FSC-H/FSC-A doublet discrimination, and SSC-H/FSC-H for cell size and cell complexity analysis. Cells were incubated with TGF-β1 (5 ng/ml) and 1 mM of IPA in non-serum media for 24 h. LX-2 cells were gated into lower left quadrants (SSC-H⁻/FSC-H⁻), upper left (SSC-H⁺/FSC-H⁻), lower right (SSC-H⁻/FSC-H⁺), and upper right quadrants (SSC-H⁺/ FSC-H.⁺) for analysis of cell size and cell cytoplasm complexity. B Analysis of cell morphology for FSC-H (forward scatter, cell size) and SSC-H (side scatter, cytoplasmic complexity) by flow cytometry (30.000 events). Data were shown as mean ± SD, n = 3 independent experiments. One-way ANOVA with Bonferroni´s post hoc test was used for statistical comparisons. *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001