Fig. 2

Analysis of cell–cell communication identifies major signaling changes in PTC and ATC patients. a Circle plots depict the global interaction strength in the cell–cell communication network from all the tested samples. b The inferred outgoing communication patterns of secreting cells (left), and the inferred incoming communication patterns of target cells (right), show the correspondence between the inferred latent patterns and cell groups, as well as signaling pathways. The thickness of the flow indicates the contribution of the cell group or signaling pathway to each latent pattern. c Bar plots showing the number of inferred interactions (left) or interaction strength (right) in the cell–cell communication network analyzed via CellChat across the PTC and ATC. d Heatmaps of the differential number of interactions between PTC and ATC, showing the outgoing and incoming signaling change of each cell group in greater detail (the top-colored bar plot represents the sum of each column of values displayed in the heatmap (incoming signaling). The right-colored bar plot represents the sum of each row of values (outgoing signaling). e Comparison of the signaling pathway based on the relative information flow between pairwise datasets. f The heatmap shows the relative importance of each cell group based on the computed four network centrality measures of the WNT signaling network. g Circle plots showing differential WNT signaling networks between PTC and ATC. ATC anaplastic thyroid cancer, PTC papillary thyroid cancer