Fig. 17

Downregulation of EMX2OS enhanced the stemness of ccRCC, whereas Downregulation of LINC00944 reduced the stemness of ccRCC. A Expression of stemness markers (CD133, EPCAM, and SOX2) were upregulated and downregulated after knockdown of EMX2OS and LINC00944, respectively. B Downregulation of EMX2OS and LINC00944 affected spheroid formation ability of ccRCC cells. C The transfection efficiency of shNC and shRNA in ccRCC organoids. D The growth status of ccRCC organoids before and after transfection with shNC and shRNA. E The expression levels of KI67 in ccRCC organoids after transfection with shNC and shRNA. F The subcutaneous tumorigenesis of CAKI1 and 769P cells in nude mice was observed following transfection with shNC and shRNA. Additionally, the variations in tumor volume and weight over a 30-day period post-treatment were documented. G The lung metastatic tumor formation of CAKI1 cells following transfection with shNC and shRNA. *P < 0.05. **P < 0.01. ***P < 0.001