Fig. 4

ATF4 drives DNM1-dependent endocytosis (DDE) to promote CD58 degradation. a CD58 expression (red) and LAMP1 organelles (green) in MHCC-97H overexpressing DNM1. Scale bar, 10 μm. b Number of LAMP1⁺ organelles in each cell. (n = 3/group). c CD58 expression (red) and EEA1 organelles (green) in MHCC-97H overexpressing DNM1. Scale bar, 10 μm. d Number of EEA1⁺ organelles in each cell. (n = 3/group). e CD58 expression (red) and LAMP1 organelles (green) in MHCC-97H overexpressing ATF4. Scale bar, 10 μm. f Number of LAMP1⁺ organelles in each cell. (n = 3/group). g CD58 expression (red) and EEA1 organelles (green) in MHCC-97H overexpressing ATF4. Scale bar, 10 μm. h Number of EEA1⁺ organelles in each cell. (n = 3/group). i CD58 expression (red) and LAMP1 organelles (green) in SNU-387 overexpressing DNM1. Scale bar, 10 μm. j Number of LAMP1⁺ organelles in each cell. (n = 3/group). k CD58 expression (red) and EEA1 organelles (green) in SNU-387 overexpressing DNM1. Scale bar, 10 μm. l Number of EEA1⁺ organelles in each cell. (n = 3/group). m CD58 expression (red) and LAMP1 organelles (green) in SNU-387 overexpressing ATF4. Scale bar, 10 μm. n Number of LAMP1⁺ organelles in each cell. (n = 3/group). o CD58 expression (red) and EEA1 organelles (green) in SNU-387 overexpressing ATF4. Scale bar, 10 μm. p Number of EEA1⁺ organelles in each cell. (n = 3/group). Statistical analysis performed using two-tailed t-test. ns for P > 0.05, *for P < 0.05, **for P < 0.01, ***for P < 0.001, ****for P < 0.0001