Fig. 8

Acetylation weakens the activity of JMJD6 in regulating METTL14 expression and affecting its mediated m6A modification to regulate SLC3A2. (A, B) Western blot (A) and RT-qPCR (B) were used to detect the effect of JMJD6 acetylation on METTL14 expression at the protein and mRNA levels, respectively. (C) CHIP assay was used to verify the regulation of JMJD6 acetylation on the enrichment level of H4R3me2a in the promoter region of METTL14. (D, E) Western blot (D) and RT-qPCR (E) were used to detect the effect of JMJD6 acetylation on SLC3A2 expression at the protein and mRNA levels, respectively. (F) MeRIP was used to detect the m6A level of SLC3A2 in JMJD6-WT, JMJD6-K375R and JMJD6-K375Q groups, and to verify the effect of JMJD6 acetylation on the m6A modification level of SLC3A2 mRNA. (G) MeRIP was used to detect the effect of METTL14 knockdown and JMJD6 mimics K375 deacetylation on the m6A modification of SLC3A2 mRNA. (H) CCK-8 was used to detect the effect of SLC3A2 knockdown and JMJD6 acetylation deletion on cell proliferation. (I, J) Cell viability (I) and ROS levels (J) were detected in H1299 cells to assess whether SLC3A2 knockdown would alter the sensitivity to erastin. Data are expressed as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001