Fig. 3

MLPH inhibits the proliferation, invasion, and migration of TNBC. a. The protein expression of MLPH protein was detected in cell lines by western blot. MLPH protein levels were quantified by normalizing to the intensity of the Actin band. Error bars represent the mean ± SEM. ^*p < 0.05, ^***p < 0.001. b. Wound-healing analysis of different subtypes breast cancer cells. Results were representative of three independent experiments. ^****p < 0.0001. c. Transwell analysis of different subtypes breast cancer cells. Results were representative of three independent experiments. ^****p < 0.0001. d and e. CCK8 proliferation analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH, the data were presented as a histogram of the mean and SEM of three independent experiments, ^****p < 0.0001. f. Colony formation analysis of HS578T or MDA-MB-231 cells stable transfected with Flag or Flag-MLPH, representative pictures of the colonies were shown. The data were presented as a histogram of the mean and SEM of three independent experiments. ^****p < 0.0001. g and h. Wound-healing analysis of MDA-MB-231(g) or HS578T(h) cells stable transfected with Flag or Flag-MLPH. Results were representative of three independent experiments. ^***p < 0.001, ^****p < 0.0001. i and j. Transwell analysis of MDA-MB-231(i) or HS578T(j) cells stable transfected with Flag or Flag-MLPH. Results were representative of three independent experiments. ^****p < 0.0001