Fig. 2

MLPH is downregulated and negatively correlated with lymph node metastasis in TNBC. a. RFS curves for the key gene MLPH of patients with/ without lymph node metastasis in the GEO databases. b. Predict the expression of MLPH in different subtypes breast cancer and adjacent cancer in TCGA database. c. Predict the protein expression of MLPH in different subtypes breast cancer and normal tissues in CPTAC database. d and e. MLPH expression levels in 80 different subtypes clinical breast tissues were determined by Western blotting with the indicated antibodies. MLPH protein levels were quantified by normalizing to the intensity of the Actin band. Error bars represent the mean ± SEM. ^*p < 0.05, ^***p < 0.001. f. Representative images of IHC staining. The boxed areas in the top images are magnified in the bottom images. Original magnification, 100×. g. MLPH expression levels in breast cancer tissues. The intensity values in (a) are expressed as the H-score. The relative staining across all samples was shown in the boxplot (t test, ^*p < 0.05, ^**p < 0.01). h. MLPH expression levels in different subtypes breast cancer tissues (with and without metastasis). The intensity values in (a) are expressed as the H-score. The relative staining across all samples was shown in the boxplot (t-test, ^*p < 0.05). i. Kaplan-Meier plot of OS or disease-free survival (DFS) of different subtypes breast cancer patients stratified by MLPH expression levels