Fig. 6

NGEF expression promotes the growth of tumor and nerve fibers. (A) Xenografts were harvested for imaging and weighing. (B&C) Tumor weight and volume were calculated at the different time points. Student’s t test and Two-way ANOVA. **P < 0.01, ***P < 0.001 as compared to vector group, ^#P < 0.01 as compared to over-NGEF + ALW-11-41-27 group. (D&E) Semiquantitative analysis of NGEF expression level by western blot. One-way ANOVA. *P < 0.05 and **P < 0.01. (F&G) Ki67 staining and semiquantitative analysis between groups. Scale bar: 20 μm. One-way ANOVA. ***P < 0.001. (H&I) PGP9.5 staining and semiquantitative analysis between groups. Scale bar: 20 μm. *P < 0.05. (J) PGP 9.5 concentration measured by ELISA and compared between groups. One-way ANOVA. **P < 0.01, ***P < 0.001. (K) Elevated NGEF expression in lung tumor cells promotes the neurogenesis and the proliferation of tumor cells via Ephrin-A3/EphA2 axis