Fig. 8

PVT1 up-regulates NRG1 by competitively binding with miR-143-3p to promote the migration and invasion of HCC. (A) qRT-PCR and WB assays were performed to analyze NRG1 expression in HCC cells after co-transfection with shPVT1 and a miR-143-3p inhibitor. (B) qRT-PCR and WB analyses were performed to assess NRG1 expression levels in HCC cells that overexpressed PVT1 and had been transfected with miR-143-3p mimics. (C, E) Wound healing and transwell experiments were conducted to evaluate the migratory and invasive potentials of HCC cells transfected with shPVT1 and miR-143-3p inhibitor (D, F) After the miR-143-3p mimics and PVT1 were transfected into HCC cells, wound healing and transwell assays performed. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001