Fig. 4

HMGCL inhibits the activation of the PI3K/AKT/mTOR signaling pathway in osteosarcoma cells. (A-B) Volcanic maps and heat maps showed significant changes in gene and expression patterns after overexpression of HMGCL. (C) KEGG analyzed the functional pathways of differential gene enrichment. (D-F) western blot analysis of the changes of PI3K/AKT/mTOR signaling pathway-related proteins after HMGCL overexpression. (G-I) western blot analysis was performed to detect the changes of PI3K/AKT/mTOR signaling pathway-related proteins changes after HMGCL silencing. ** P < 0.01, *** P < 0.001