Fig. 4

Synergistic regulation of regulatory T cells by E. coli and PD-1. A Flow cytometry results of CD3⁺CD4⁺CD25⁺Foxp3⁺ T cells in tumor tissues, spleen and peripheral blood after different treatments. B–D Quantitative analysis of CD3⁺CD4⁺CD25⁺Foxp3⁺ regulatory T cells (n = 4). E Immunofluorescence staining of Tregs cells in tumor (labeled with red. Scale bars: 40 µm). F Normalized relative fluorescence intensity. (n = 4, ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, calculated by one-way ANOVA)