Fig. 4

Cell-state transition trajectory inference of CAFs. A UMAP plot demonstrating possible differentiation trajectories between the three fibroblasts. B UMAP plot demonstrating the distribution of fibroblasts in pseudotime. C Violin plot demonstrating the comparison of the three fibroblasts in pseudotime. D Distribution of the three fibroblasts as well as fibroblasts in normal and tumour tissues in pseudotime. E Heatmap demonstrating genes in fibroblasts during their differentiation over time and clustering analysis dividing them into three modules: progenitor, activation and differentiation. F Trends in gene expression of the three modules over time. G Trends in gene expression of the three modules over time in normal and tumour tissues. H Hypoxia, TGFb, VEGF, WNT pathway scores over time in normal and tumour tissues