Fig. 6

Differentiation of GSCs into endothelial cells. A, B violin plots showing the normalized expression levels of signature genes across the identified cell types. C Dot plots showing intercellular ligand and receptor pairs between endothelial and other cell types. D Spatial heatmaps of PTN and PTPRZ in patient UKF423. E The t-SNE plot of 9 identified cell types in GBM. F Pseudotime analysis of single-cell RNA-seq data. G The correlation heatmap illustrates the relationship between pairs of cell types infiltrated in GBM. The color is scaled by the correlation value. H Representative multiplex immunofluorescence images of CD31 (pink) and CD44 (red) in GBM. Scale bar represents 20 μm