Fig. 8
YSV induced cell cycle arrest and apoptosis of OSCC by regulating PTEN/PI3K/AKT signaling pathway in vitro. (a, b) The proliferation curve of SCC-15 cells (a) and CAL-27 cells (b) after cells were treated with YSV, PI3K-IN-1, LY294002 or the combination usage of YSV and PI3K-IN-1 or LY294002; (c, d) Distribution (c) and statistical analysis (d) of cell cycles of SCC-15 cells (up) and CAL-27 cells (bottom), respectively, treated with LY294002, YSV and the two combined usage; (e, f) Representative flow cytometry plots (e) and statistical analysis (f) of SCC-15 cells (up) and CAL-27 cells (bottom), respectively, treated with LY294002, YSV and the two combined usage; (g) The protein expression of CDK1, Cyclin B1, Bcl-2, Bax, Cleaved caspase-3, PTEN, PI3K, p-PI3K, AKT and p-AKT in SCC-15 cells (left) and CAL-27 cells (right), treated with LY294002, YSV and the two combined usage. *, #, & P < 0.05; **, ##, && P < 0.01; n = 3. Asterisks represent the differences between G1 phase or the apoptosis rete, pounds represent the differences between S phase; ampersands represent the differences between G2/M phase