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Table 2 Subgrouping of HUVECs after transfection

From: MT2A promotes angiogenesis in chronically ischemic brains through a copper–mitochondria regulatory mechanism

Groups

Procedures

Control group

Treated with ES (1 × 10− 3 M) only

CPO group

Treated with ES (1 × 10− 3 M) and CuCl2 (1 µM)

CPOMT2A+ NC group

Transfected with the control vector of pcDNA and treated with ES (1 × 10− 3 M) and CuCl2 (1 µM)

CPOMT2A+ group

Transfected with pcDNA-MT2A and treated with ES (1 × 10− 3 M) and CuCl2 (1 µM)

CPOMT2A− NC group

Transfected with the control scramble siRNA and treated with ES (1 × 10− 3 M) and CuCl2 (1 µM)

CPOMT2A− group

Transfected with siRNA-MT2A and treated with ES (1 × 10− 3 M) and CuCl2 (1 µM)

  1. HUVECs: human umbilical vein endothelial cells; ES: elesclomol; CPO: copper overload; MT2A: metallothionein 2 A; NC: negative control