Fig. 9
Effects of CPO on angiogenesis in the brains of 2VO + EMS rats. (A) Column chart showing the copper concentration in brain tissue from different groups. (B) Representative western blot showing the expression of DLAT and CD31 normalized to the expression of β-actin in different groups. (C) Densitometric analyses of oligomer-DLAT and CD31 expression normalized to the expression of β-actin in different groups. (D) Column chart showing the changes in DLAT and CD31 mRNA expression in brain tissue from rats. (E) Immunofluorescence staining showing DLAT (green) and CD31 (red) expression. Scale bar = 20 μm. (F) CD31 was segmented and quantified in each condition. The error bars represent the ± SDs. ns: P > 0.05, *: P < 0.05, **: P < 0.01. CPO: copper overload; 2VO: 2-vessel occlusion; EMS: encephalo-myo-synangiosis; SD: standard deviation