Fig. 8

MT2A relieves the impairment of mitochondrial structure and function caused by CPO in HUVECs. (A) TEM results showing changes in the mitochondrial structure in the different groups. The white arrows indicate normal mitochondria. The orange arrows indicate swelling. The yellow arrows indicate vacuoles. White scale bar = 1 μm. Yellow scale bar = 0.5 μm. (B) Quantification of the mitochondrial number, abnormal mitochondrial percentage, and average mitochondrial minor axis length. (C) JC-10 results showing the mitochondrial function of HUVECs in different groups. (D) Column chart showing the ratio of aggregated JC-10 to monomeric JC-10 in the different groups. The error bars represent the ± SDs. ns: P > 0.05, *: P < 0.05, **: P < 0.01. HUVEC: human umbilical vein endothelial cell; CPO: copper overload; TEM: transmission electron microscopy; MT2A: metallothionein 2 A; SD: standard deviation