Fig. 7

The effect of MT2A on the cuproptosis process induced by CPO. (A) Representative western blot showing the expression of DLAT, SDHB and HSP70 normalized to the expression of β-actin in different groups. (B) Densitometric analyses of DLAT, SDHB and HSP70 expression normalized to the expression of β-actin in different groups. (C) Immunofluorescence staining showing DLAT (green) and MitoTracker (red). Scale bar = 20 μm. (D) Quantitative analysis of immunostaining of DLAT foci in different groups. The error bars represent the ± SDs. *: P < 0.05, **: P < 0.01. HUVEC: human umbilical vein endothelial cell; CPO: copper overload; MT2A: metallothionein 2 A; SD: standard deviation