Fig. 6

Effects of MT2A on the viability of HUVECs under CPO. (A) Column chart showing the copper content of HUVECs in different group. (B) Column chart showing the viability of HUVECs in different groups at 48 h via the CCK-8 assay. (C) Representative fields showing the results from the tube formation assays in different groups. Bar = 100 μm. The results were quantified by (D) the total number of tubes, nodes, branches and meshes. (E) Representative fields showing the results from the scratch wound assays at 48 h obtained for each group. Bar = 200 μm. (F) The results were quantified by the percentage of wound closure at 48 h. (G) Representative fields showing the results from the Transwell migration assays for each group. Bar = 50 μm. (H) The results were quantified by the invaded cells per field. The error bars represent the ± SDs. ns: P > 0.05, *: P < 0.05, **: P < 0.01, **: P < 0.001. HUVEC: human umbilical vein endothelial cell; CPO: copper overload; ES: elesclomol; MT2A: metallothionein 2 A; SD: standard deviation