Fig. 2

Procedures used for modeling 2VO + EMS rats. (A, B) Procedures used for CCA ligation. The white arrows indicate the CCA. (C) Representative images showing a rat brain after opening the skull and dura. (D) Rats were subjected to IV lentivirus injection to regulate the expression level of MT2A. (E) Brain samples showing the covering of the TM in the ischemic brain (black arrow). (F) Representative graph of CBF changes before and after 2VO via LDF. 2VO: 2-vessel occlusion; EMS: encephalo-myo-synangiosis; CCA: common carotid artery; MT2A: metallothionein 2 A; TM: temporal muscle; CBF: cerebral blood flow; IV: intraventricular