Fig. 1
Isolation of RBD-directed memory B cells by cell sorting. (a) PBMCs gated on total live CD3−CD8−CD14−CD19+CD20+CD27+IgG+RBD+B cells were sorted from convalescent patients YYQ, SW and LQL, respectively. (b) Chord diagram representation of V and J frequency of isolated antibodies from YYQ, SW and LQL, respectively. The size of the inner segments corresponds to the number of sequences of each gene, and the color-coded diagrams indicate the pairing network of the VH and VJ. (c) SHM rates and CDR3 lengths of VH and Vκ/λ of SW, YYQ and LQL, respectively