Fig. 6

HDAC11 expression affects intracellular survival of S. aureus via IL10/mTOR. (A, B, and C) Detection of HDAC11 and H3K9ac protein levels in BMDMs overexpressing HDAC11 using Western blot. GAPDH is used as a reference protein to quantify HDAC11 expression, and H3 to quantify H3K9ac expression. (D) Validation of IL10 production levels induced by 24-hour intracellular S. aureus infection in BMDMs overexpressing HDAC11, using ELISA. (E and F) Bacterial plating and (G and H) flow cytometry to assess the impact of the mTOR inhibitor Rapamycin on intracellular bacterial load in BMDMs overexpressing HDAC11, with or without inhibitor treatment. Quantitative analysis of intracellular bacterial counts at 6 h and 24 h, and the proportion of BMDMs infected with GFP-S. aureus. Statistical analysis was performed using a two-tailed Student’s unpaired t-test: ns, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001. The data are presented as the mean ± SD from at least three independent experiments