Fig. 5

S. aureus infection results in the downregulation of HDAC11 in macrophages, affecting the regulation of IL10 and mitophagy. (A) Heatmap of the top 10 differential protein expressions increased or decreased in BMDMs 24 h after intracellular infection with S. aureus. (B, C, and D) Western blotting analysis of HDAC11 and H3K9ac protein expression levels at various times after intracellular infection of S. aureus (MOI = 10). β-tubulin is used as a reference protein for quantifying HDAC11 expression, and H3 for H3K9ac. (E) ELISA quantitative analysis of IL10 levels in the supernatant of BMDMs infected with S. aureus (MOI = 10) at various time points. (F, G, H and I) Western blotting analysis of LC3, P62, mTOR, and p-mTOR protein expression levels at various time points during intracellular infection. GAPDH is used as a reference protein to quantify P62 expression; changes in LC3ii/LC3i and p-mTOR/mTOR are also quantified. (J, K, and L) Preparation of mitochondria (Mito) and Western blotting analysis of Pink-1 and Parkin protein expression levels at various time points during intracellular infection. COX IV is used as a reference protein to quantify Pink-1 and Parkin expression levels. Statistical analysis was performed using one-way ANOVA (C, D, G, H, I, K, L) or two-tailed Student’s unpaired t-test (E): ns, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001. The data represent the mean ± SD from at least three independent experiments