Fig. 4

Scutellarin@BSA inhibited EndMT by inhibiting JAK1-STAT1 signaling. (A-D) To assess changes in endothelial and mesenchymal cells, liver sections from control mice and mice from the MASH model group treated with Scutellarin@BSA were stained and analyzed for the endothelial cell marker CD31 (red) and the mesenchymal cell markers α-SMA (green) and FSP1 (green). After staining, these markers were assessed quantitatively (n = 4 mice/group). Scale bar, 100 μm. (E-H) Scutellarin@BSA effectively inhibited the activation of the STAT1 protein in liver tissue. Liver tissue extracts were subjected to various therapeutic evaluated using WB (n = 4 mice/group). Protein expressions were qualified with densitometry analysis using ImageJ. Statistical analyses were performed using GraphPad Prism 9 software and values across groups were compared using one-way ANOVA. Data are expressed as the mean ± S.D. *p < 0.05; **p < 0.01