Fig. 9
(A) Western blot analysis showing DNASE1L3 expression levels across various cell lines. (B) Confirmation of DNASE1L3 overexpression in PANC-1 and Mia PaCa-2 cells via Western blot. (C) CCK-8 assay measuring the proliferation of PDAC cells with DNASE1L3 overexpression compared to NC-transfected PDAC cells. (D) Representative images and quantification of the colony formation assay for PANC-1 and Mia PaCa-2 cells with DNASE1L3 overexpression and siNC. Magnification: 200×. (E) Transwell migration assay showing the effect of DNASE1L3 on the migration ability of PDAC cells. Magnification: 200×. (F) Top three signaling pathways identified by GSEA analysis of DNASE1L3. (G) Heatmap of cytokine levels in the culture supernatants of PDAC cells with DNASE1L3 overexpression and NC. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001)