Fig. 7

Dietary selenium supplementation rescues γδT loss and ameliorates lupus symptoms in Lpr mice. (A) Lpr mice were administered drinking water supplemented with selenium at concentrations of 2 mg/L and 4 mg/L for 8 weeks, respectively. Se, selenium. (B) Representative flow cytometry plots and quantification of γδT in CD3 + T cells from Mpj and Lpr mice with and without dietary selenium supplementation. (C and D) Proteinuria (C) and ANA (D) from Mpj and Lpr mice with and without dietary selenium supplementation. ANA, anti-nuclear antibodies. (E and F) Representative plot and quantification of Zo-1 expression based on immunohistochemical staining. MFI, Mean Fluorescence Intensity; Zo-1, zona occludens-1. (G) Representative plot and quantification of H&E staining, IgG and C3 deposition in the kidneys. Statistical significance in (B-D, F-G) was determined by one-way ANOVA with dunnett post hoc test. Each dot denotes an individual mouse. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001