Fig. 3
From: Usp7 contributes to the tail regeneration of planarians via Islet/Wnt1 axis
Cell apoptosis in regenerating planarians after Usp7 RNAi. A Immunostaining with whole-mount TUNEL of the regenerating trunk fragments. B Apoptosis density in regenerating trunk fragments. Vertical bars represent the mean ± SD (n = 6). Scale bar: 100 μm. C The mRNA expression level of caspase3 in regenerating trunk fragments after Usp7 RNAi for R 3 d and R 7 d. Vertical bars represent the mean ± SD (n = 3). D The expression level of Cleaved-Caspase3 and BCL2 in the trunk fragments after Usp7 RNAi for R 3 d and R 7 d by Western blot. Asterisks indicate statistical differences (*p < 0.05; **p < 0.01; ***p < 0.001)