Table 1 Primer sequence
Gene | Primer sequence |
|---|---|
MIR4435-2HG-F | 5’- CGGAGCATGGAACTCGACAG -3’ |
MIR4435-2HG-R | 5’- CAAGTCTCACACATCCGGGC -3’ |
CD44-F | 5’- CACACCCTCCCCTCATTCAC -3’ |
CD44-R | 5’- CAGCTGTCCCTGTTGTCGAA -3’ |
KLF4-F | 5’- GTCCCGGGGATTTGTAGCTC-3’ |
KLF4-R | 5’- CGTCTTCCCCTCTTTGGCTT-3’ |
OCT4-F | 5’- AAACCCACACTGCAGCAGATCA -3’ |
OCT4-R | 5’- CCCCTGAGAAAGGAGACCCA -3’ |
SOX2-F | 5’- CATGAAGGAGCACCCGGATT -3’ |
SOX2-R | 5’- TTCATGTGCGCGTAACTGTC -3’ |
miR-1252-5p-RT | 5’- gAAAgAAggCgAggAgCAgATCgAggAAgAAgACggAAgAATgTgCgTCTCgCCTTCTTTCTAAATgAA -3’ |
miR-1252-5p-F | 5’- ggTggAgggAgAAggAAATTgAA -3’ |
U6-F | 5’- CTCgCTTCggCAgCACA -3’ |
U6-R | 5’- AACgCTTCACgAATTTgCgT -3’ |
STAT1-F | 5’- TCTGGAAAACGCCCAGAGATT -3’ |
STAT1-R | 5’- CTACTTCCTCTGTTCTGCAAGG -3’ |
GAPHD-F | 5’- CAAATTCCATGGCACCGTCA -3’ |
GAPHD-R | 5’- TGATGACCCTTTTGGCTCCC -3’ |