Fig. 7

Enforced expression of STAT1 promotes pancreatic cancer stemness.(A) RT-qPCR analysis of STAT1 expression in cells transfected with miR-1252-5p inhibitor or mimic. (B) RT-qPCR analysis of STAT1 expression in cells with MIR4435-2HG knockdown or overexpression. (C) Representative images of CSC spheres in cells with STAT1 overexpression. (D) Colony formation assay and quantification in BxPC-3 and PANC-1 cells with STAT1 overexpression. (E) Western blot analysis of STAT1 and phospho-STAT1 expression in MIR4435-2HG knockdown cells, miR-1252-5p mimic cells, MIR4435-2HG overexpressing cells, and miR-1252-5p inhibitor-transfected cells. (F) RT-qPCR analysis of CD44, KLF4, OCT4, and SOX2 expression in cells with STAT1 overexpression. (G) Western blot analysis of phospho-STAT1, CD44, KLF4, OCT4, and SOX2 expression in cells co-transfected with MIR4435-2HG and miR-1252-5p mimic, or in MIR4435-2HG-overexpressing cells treated with fludarabine. (H) Western blot analysis of phospho-STAT1, CD44, KLF4, OCT4, and SOX2 expression in MIR4435-2HG knockdown cells with enforced STAT1 expression. *p<0.05, **p<0.01, ***p<0.001