Skip to main content
Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Long non-coding RNA MIR4435-2HG modulates pancreatic cancer stem cells and chemosensitivity to gemcitabine by targeting the miR-1252-5p/STAT1

Fig. 5

MIR4435-2HG targets miR-1252-5p to promote pancreatic cancer stemness.(A) RT-qPCR analysis of miR-1252-5p expression in cells with MIR4435-2HG knockdown and overexpression. (B) RT-qPCR analysis of miR-1252-5p expression in BxPC-3 and PANC-1 cells transfected with miR-1252-5p inhibitor or mimic. (C) Representative images of cancer stem cell (CSC) spheres in cells transfected with miR-1252-5p mimic or inhibitor. (D) RT-qPCR and (E) Western blot analyses of CD44, KLF4, OCT4, and SOX2 expression in cells with miR-1252-5p inhibition. (F) Colony formation assay and quantification of colony numbers in BxPC-3 and PANC-1 cells transfected with miR-1252-5p mimic or inhibitor. *p<0.05, **p<0.01, ***p<0.001

Back to article page

Journal of Translational Medicine

ISSN: 1479-5876

Contact us