Skip to main content
Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Long non-coding RNA MIR4435-2HG modulates pancreatic cancer stem cells and chemosensitivity to gemcitabine by targeting the miR-1252-5p/STAT1

Fig. 3

Overexpression of MIR4435-2HG enhances pancreatic cancer stemness.(A) RT-qPCR analysis of MIR4435-2HG expression in BxPC-3 and PANC-1 cells following transfection with either an empty vector (control) or MIR4435-2HG-overexpression vector. (B) Representative images of cancer stem cell (CSC) spheres in control and MIR4435-2HG-overexpressing cells. (C) RT-qPCR and (D) Western blot analyses of CD44, KLF4, OCT4, and SOX2 expression in control and MIR4435-2HG-overexpressing cells. (E) Colony formation assay and quantification of colony numbers in BxPC-3 and PANC-1 cells with MIR4435-2HG overexpression. *p<0.05, **p<0.01, ***p<0.001

Back to article page

Journal of Translational Medicine

ISSN: 1479-5876

Contact us