Fig. 3

PTL inhibited mitochondrial mediated apoptosis by up-regulating BCL-xL. A PTL chemical structure. B Network pharmacology analysis of PTL target. C Venn diagram screens PTL action on mitochondrial-associated genes, PTL: PTL target, MITO: Mitochondrial-associated genes, UP: Up-regulation differential genes in LPS vs Nor, DOWN: Down-regulation differential genes in LPS vs Nor. D WB detection of PTL’s effect on BCL-xL expression (n = 3 independent experiments). E WB detection of the interference efficiency of shBCL-xL (n = 3 independent experiments). F, G Knocking down BCL-xL’s effect on mitochondrial morphological structure after being treated with PTL (bar = 10 μm), each group randomly selected 10 cells and the mitochondrial morphology was blindly scored and classified into two categories: Long (> 3 µm), Short (≤ 3 µm). H, I Knocking down BCL-xL’s effect on MMP after being treated with PTL (bar = 40 μm) (n = 3 independent experiments). J, K Knocking down BCL-xL’s effect on ROS after being treated with PTL (bar = 100 μm) (n = 3 independent experiments). L, M Knocking down BCL-xL’s effect on cell apoptosis, as detected by TUNEL (bar = 20 μm) and Annexin V (bar = 100 μm), after treatment with PTL (n = 3 independent experiments). N Knocking down BCL-xL’s effect on VECs function after being treated with PTL (n = 3 independent experiments). O WB detection of PTL’s effect on BCL-xL expression in vivo (n = 3 independent experiments). P WB detection of A-133 interference with BCL-xL (n = 3 independent experiments). Q The effect of BCL-xL inhibitor on the bleeding time of septic rats (n = 6 rats each group). R, S The effect of BCL-xL inhibitor on the change in the number of VECs (bar = 40 μm) (n = 6 rats each group). a: P < 0.05 as compared with the LPS + PTL or Sepsis + PTL group