Fig. 2

The ability of HPPS-AP@RMn to target and activate DCs in vitro.(A) Represent images of confocal imaging to evaluate the ability of HPPS-AP@RMn to target DCs.The scale bar is 100 μm. (B-D) Expression levels of CD80 (B), CD86 (C) and MHC-II (D) in BMDCs measured by flow cytometry. LPS was used as a positive control (n = 3). (E) Protein expression levels of p-TBK1, TBK1, p-IRF3, IRF3, and β-actin in BMDCs after different treatments detected by Western blotting. (F-H) Concentrations of IFN-α (F), IFN-β (G) and TFN-α (H) in BMDC culture supernatants (n = 6). Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; NS, not significant